Nearly all of self-assembled fluorescent dyes endure from aggregation-caused quenching (ACQ), which detrimentally impacts their diagnostic and therapeutic effectiveness. Whereas aggregation-induced emission (AIE) lively dyes provide a promising answer to beat this limitation, they could face vital challenges because the intracellular setting typically prevents aggregation, resulting in disassembly and posing challenges for AIE fluorogens. Current progress in sign amplification by the disassembly of ACQ dyes has opened new avenues for creating ultrasensitive optical sensors and enhancing phototherapeutic outcomes. These advances are well-aligned with cutting-edge applied sciences corresponding to single-molecule microscopy and focused molecular therapies. This work explores the idea of disaggregation-induced emission (DIE), showcasing the revolutionary capabilities of DIE-based dyes from their design to their software in sensing, bioimaging, illness monitoring, and remedy in each mobile and animal fashions. Our goal is to offer an in-depth comparability of aggregation versus disaggregation mechanisms, aiming to stimulate additional developments within the design and utilization of ACQ fluorescent dyes by DIE expertise. This initiative is poised to catalyze scientific progress throughout a broad spectrum of disciplines.
